A.                Positions and Honors.

Positions and Employment

1985 – 1988                Resident, Department of Anesthesiology, Beijing Hospital, Beijing

1989 – 1995                MS and PhD Graduate Student, Pharmacology, Southern Illinois University

1996 – 1999                NIH Postdoctoral Fellow, Anesthesiology, Mayo Clinic, Rochester, Minnesota

2000 – 2006                Assistant Professor (Tenure-Stream), Department of Pharmacology and Toxicology and the Neuroscience Program, Michigan State University

8/2003 – 2006             Assistant Prof, Dept of Neurology and Ophthalmology, Michigan State University

10/2005 – 2006           Assistant Prof, Cell and Molecular Biology Program, Michigan State University

2007 – Present            Associate Professor (with tenure), Michigan State University

Study Sections, Editorial Boards and Professional Memberships

2/2001 – Present      Executive Committee, Division for Systems and Integrative Pharmacology, American Society of Pharmacology and Experimental Therapeutics (ASPET)

1/2002 – 4/2005       Member, AHA National Brain and Stroke 2 Peer Review Study Section

2/1998 – Present      Fellow, AHA Stroke Council, American Heart Association

9/2002 – Present      Fellow, AHA Council for High Blood Pressure Research

7/2004 – Present      Executive Committee Member, Division of Cardiovascular Pharmacology, American Society of Pharmacology and Experimental Therapeutics (ASPET)

1/2005 – Present      North American Editor, Acta Pharmacologica Sinica

6/2005 – Present      Editorial board member, Journal of Cardiothoracic and Renal Research

10/2005 – Present    Editorial board member, Atherosclerosis, Thrombosis and Vascular Biology

2/2006 – Present      National Merit Review Panel, Veterans Health Administration, US Department of Veterans Affairs

1/2007 – Present      Editorial board member, American Journal of Physiology (Heart & Circ Physiology)

2/2007 – Present      Editor for Diabetes and Obesity/Metabolic, Clinical and Experimental Pharmacology and Physiology

2/2007 – Present      Member, AHA National Vascular Biology Blood Pressure/Regulation 2 Study Section

6/2007 – Present      Member, American Diabetes Association (ADA) National Study Section

1/2008– Present       Member, American Heart Association (AHA) Brain 1 Peer Review Committee

Honors

11/1985           Physician Training Award, Chinese Medical Association/Beijing Affiliate

07/1994           Graduate Student Travel Award, XIIth International Congress of Pharmacology (IUPHAH), American Society for Pharmacology and Experimental Therapeutics (ASPET), Montreal

1996 – 1999    NIH Postdoctoral Fellowship, Department of Anesthesiology, Mayo Clinic, Rochester, MN

04/1997           Caroline tum Suden/Frances A. Hellbrandt Professional Opportunity Award, American Physiological Society (APS)

06/1997           Bursary Award, Brain’97, International Society of Cerebral Blood Flow and Metabolism

07/1997           Bursary Award for Symposium on Mechanisms of Cerebrovascular Function and Regulation, International Society of Cerebral Blood Flow and Metabolism, Williamsburg, Virginia

07/1998             James A. Bain Young Scientists Travel Award, XIIIth International Congress of Pharmacology (IUPHAR), ASPET, Munich, Germany

08/2001           Travel Award, the XXXIV International Union of Physiological Sciences (IUPS), American Physiology Society (APS), Christchurch, New Zealand

07/2002           James A. Bain Young Scientists Award, XIVth International Congress of Pharmacology, American Society for Pharmacology and Experimental Therapeutics (ASPET), San Francisco

11/2003           Young Investigator Travel Award, AHA Council on Basic Cardiovascular Sciences, Orlando

5/2007             Chair, “Nitric oxide deficiency and cardiovascular disease”, EB07, Washington DC, April 2007

11/2007           Chair, “Angiogenesis IV”, AHA Scientific Sessions 2007, Orlando, FL, November 6, 2007

B.                 Selected peer-reviewed publications (excluding abstracts and book chapters).

1.        Chen, FY, Lee, TJ-F. Role of nitric oxide in neurogenic vasodilation of porcine cerebral artery. Journal of Pharmacology and Experimental Therapeutics 265: 339-345, 1993.

2.        Chen, FY, Lee, TJ-F. Arginine synthesis from citrulline in perivascular nerves of cerebral artery. Journal of Pharmacology and Experimental Therapeutics 273: 895-901, 1995.

3.        Lee TJ-F, Sarwinski S, Ishine T, Lai C-C, Chen FY. Glutamine inhibits cerebral neurogenic vasodilation. Journal of Pharmacology and Experimental Therapeutics, 276: 353-358, 1996.

4.        Chen AFY, Jiang S, Crotty TB, Tsutsui M, Smith LA, O’Brien T, Katusic ZS. Effects of in vivo adventitial expression of recombinant endothelial nitric oxide synthase gene in cerebral arteries. Proceedings of National Academy of Science of USA 94, 12568-12573, 1997.

5.        Chen AFY, O’Brien T, Tsutsui M, Kinoshita H, Pompili VJ, Crotty TB, Spector DJ, Katusic ZS. Expression and function of recombinant endothelial nitric oxide synthase gene in canine basilar artery. Circulation Research 80, 327-335, 1997.

6.        Chen AFY, O’Brien T, Katusic ZS. Transfer and expression of recombinant nitric oxide synthase genes in cardiovascular system. Trends in Pharmacologic Sciences 19, 276-286, 1998.

7.        Tsutsui M, Chen AFY, O’Brien T, Crotty TB, Katusic ZS. Adventitial expression of recombinant nitric oxide synthase gene restores nitric oxide production in arteries without endothelium. Arteriosclerosis, Thrombosis, and Vascular Biology 18, 1231-1241, 1998.

8.        Shah VH, Chen AF, Cao S, Hendrickson H, Weiler D, Smith LA, Yao J, Katusic ZS.  Gene transfer of recombinant endothelial nitric oxide synthase to liver in vivo and in vitro. American Journal of Physiology (Gastrointestinal Physiology) 279, G1023-G1030, 2000.

9.        Xu H, Fink GD, Chen AF, Watts SW and Galligan JJ. Nitric oxide-independent effects of tempol on sympathetic nerve activity and blood pressure in normotensive rats. American Journal of Physiology (Heart and Circulatory Physiology) 281, H975-H980, 2001.

10.    Li LX Crockett E, Wang DH, Galligan JJ, Fink GD and Chen AF.  Gene transfer of endothelial NO synthase and manganese superoxide dismutase on arterial vascular cell adhesive molecule-1 expression and superoxide production in deoxycorticosterone acetate-salt hypertension. Arteriosclerosis, Thrombosis and Vascular Biology 22, 249-255, 2002.

11.    Brown RA, Ilg KJ, Chen AF, Ren J. Dietary Mg²⁺ supplementation restored endothelium-independent vasorelaxation and agonist-induced vasoconstriction in rat aorta following chronic ethanol ingestion. European Journal of Pharmacology 442, 241-250, 2002.

12.    Chen AF, Ren J, Miao CY. Nitric oxide synthase gene therapy for cardiovascular disease. (Invited Review) Japanese Journal of Pharmacology (now Journal of Pharmacological Sciences) 89, 327-336, 2002.

13.    Luo, JD, Chen, AF. Perspectives on the cardioprotective effects of statins. Current Medicinal Chemistry 10, 1241-1253, 2003.

14.    Zhang X, Ye G, Duan J, Chen AF, Ren J. Influence of gender and age on cardiac contractile function in isolated ventricular myocytes from calmodulin-induced transgenic diabetic mice. Endocrine Research 29, 227-236, 2003.

15.    Li LX, Fink GD, Watts SW, Galligan JJ, Pagano PJ, Chen AF. ET-1 Increases Vascular Superoxide via ET_A-NADPH Oxidase Pathway in Low Renin Hypertension. Circulation 107, 1053-1058, 2003.

16.    Li LX, Galligan JJ, Fink GD, Chen AF. Vasopressin enhances vascular superoxide production by activating Endothelin-1 in mineralocorticoid hypertension. Hypertension 41 [part 2], 663-668, 2003.

17.    Li LX, Watts SW, Galligan JJ, Fink GD, Chen AF. NADPH oxidase-derived superoxide augments endothelin-1-induced venoconstriction in mineralocorticoid hypertension. Hypertension 42: 316-321, 2003.

18.    Zheng JS, Yang XQ, Lookingland KJ, Fink GD, Hesslinger C, Kapatos G, Kovesdi I, Chen AF. Gene transfer of human guanosine 5’-triphosphate cyclohydrolase I restores vascular tetrahydrobiopterin level and endothelial dysfunction in low renin hypertension. Circulation 108, 1238-1245, 2003

19.    Li LX, Chu Y, Fink GD, Engelhardt JF, Heistad DD, Chen AF. Endothelin-1 stimulates arterial vascular cellular adhesion molecule-1 expression via superoxide in mineralocorticoid hypertension. Hypertension 42, 997-1003, 2003.

20.    Ren J, Zhang XC, Scott GI, Esberg LB, Ren BH, Culver BW, Chen AF. Adenovirus gene transfer of recombinant endothelial nitric oxide synthase enhances contractile function in ventricular myocytes. Journal of Cardiovascular Pharmacology 43, 171-177, 2004.

21.    Xu FP, Chen MS, Wang YZ, Yi Q, Lin S, Chen AF, Luo JD. Leptin induces hypertrophy via endothelin-1- reactive oxygen species pathway in cultured neonatal rat cardiomyocytes. Circulation 110: 1269-1275, 2004.

22.    Luo JD, Wang YY, Fu WL, Wu J, Chen AF. Gene therapy of eNOS and MnSOD accelerates delayed wound healing in type 1 diabetic mouse. Circulation 110, 2484-2493, 2004.

23.    Song WZ, Chen AF, Wang DH. Increased salt sensitivity induced by sensory denervation: role of superoxide. Acta Pharmacologica Sinica 25, 1626-1632, 2004.

24.    Wu J, Ma B, Yi S, Wang Z, He W, Luo G, Chen X, Wang X, Chen AF, Barisoni D. Comparison of multiple gene expression between hypertrophic scar and normal skin tissue by means of cDNA microarrays. Journal of Trauma 57, 1276-1286, 2004.

25.    Zhao HB, Wang SZ, He QH, Yuan L, Chen AF, Lin ZB. Ganoderma total sterol (GS) and GS(1) protect rat cerebral cortical neurons from hypoxia/reoxygenation injury. Life Sciences 76, 1027-1037, 2005.

26.    Luo JD, Chen AF. Nitric oxide: a newly discovered function on wound healing. (Invited review) Acta Pharmacologica Sinica 26, 259-264, 2005.

27.    Wang H, Chen AF, Watts SW, Galligan JJ, Fink GD. Endothelin in the splanchnic vascular bed of DOCA-salt hypertensive rats. American Journal of Physiology (Heart and Circulatory Physiology) 288, H729-H736, 2005.

28.    Zheng JS, Tang LL, Zheng SS, Zhan RY, Zhou YQ, Chen AF. Gene therapy of glial cell line-derived neurotrophic factor in a rat model of late stage Parkinson’s disease. Molecular Brain Research 134, 155-161, 2005.

29.    Cao YN, Kuwasako K, Kato J, Yanagita T, Tsuruda T, Kawano J, Nagoshi Y, Chen AF, Wada A, Suganuma T, Eto T, Kitamura K. Beyond vasodilation: The antioxidant effect of adrenomedullin in Dahl salt-sensitive rat aorta. Biochemical Biophysical Research Communication 332, 866-872, 2005.

30.    Chen AF. Cerebral vasospasm – advances in research and treatment (Invited Book Review). Journal of Neurosurgery 103, 575, 2005.

31.    Wang YP, Chen AF, Wang DH. ET_A receptor blockade prevents renal dysfunction in salt-sensitive hypertension induced by sensory denervation. American Journal of Physiology (Heart and Circulatory Physiology) 289, H2005-H2011, 2005.

32.    Kidd, GA, Hong H, Majid A, Kaufman DI, Chen AF. Inhibition of brain GTP cyclohydrolase I and tetrahydrobiopterin attenuates cerebral infarction via reducing iNOS and peroxynitrite in ischemic stroke. Stroke 36, 2705-2711, 2005.

33.    Liu HQ, Wei XB, Sun R, Cai YW, Lou HY, Wang JW, Chen AF, Zhang XM. Angiotensin II stimulates intercellular adhesion molecule-1 via an AT1 receptor/nuclear factor-кB pathway in brain microvascular endothelial cells. Life Sciences 78, 1293-1298, 2006.

34.    Thakali KM, Lau Y, Fink GD, Galligan JJ, Chen AF, Watts SW. Mechanisms of hypertension induced by nitric oxide (NO) deficiency: focus on venous function. Journal of Cardiovascular Pharmacology 47, 742-750, 2006.

35.    Chen YF, Chen H, Hoffmann A, Cool DR, Diz DI, Chappell, MA, Chen AF, Morris M. Adenovirus mediated small interference RNA for silencing angiotensin AT1a receptors in mouse brain. Hypertension 47, 230-237, 2006.

36.    Hong H. Zeng JS, Kreleun DL, Kaufman DI, Chen AF. Atorvastatin protects against cerebral infarction via inhibiting NADPH oxidase-derived superoxide in ischemic stroke. American Journal of Physiology (Heart and Circulatory Physiology) 291, H2210-H2215, 2006.

37.    Wang YP, Chen AF, Wang DH. Enhanced oxidative stress in kidneys of salt-sensitive hypertension: role of sensory nerves. American Journal of Physiology (Heart & Circ Physiol) 291, H3136-H3143, 2006.

38.    Chen DD, Chen AF. CuZn-SOD deficiency: a culprit of accelerated vascular aging process. (Invited Editorial Commentary) Hypertension 48, 1026-1028, 2006.

39.    Du YH, Chen AF. A “love triangle” elicited by electrochemistry amongst brain, heart and kidney – complex interactions between cardiac sympathetic afferent and chemoreceptor reflexes. (Invited Editorial) Journal of Applied Physiology 102, 9-10, 2007.

40.    Cui CM, Chen AF, Jiang ZP, Wu QQ, Lin JW, Wen HM, Zeng JS. NAD(P)H oxidase inhibition reduces brain fibronectin expression in stroke-prone renovascular hypertensive rats. Clinical and Experimental Pharmacology and Physiology 34, 304-309, 2007.

41.    Zhu XL, Chen AF, Lin ZB. Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice. Journal of Ethnopharmacology 111, 219-226, 2007.

42.    Krishnamurthy RG, Zemke D, Senut, MC, Frenkel MB, Chen AF, Gupta R, Majid A. Carnosine is neuroprotective against permanent focal cerebral ischemia in mice. Stroke 38, 3023-3031, 2007.

43.    Liao SJ, Lin L, Zeng JS, Huang RX, Chen AF. Endothelial-targeted transgenic GTP-cyclohydrolase I overexpression inhibits neointimal formation in mouse carotid artery. Clinical and Experimental Pharmacology and Physiology 34, 1260-1266, 2007.

44.    Yang XQ, Wang YY, Chen AF. Increased superoxide contributes to enhancement of vascular contraction in Ins2^Akita diabetic mice, an autosomal dominant mutant model. Clinical and Experimental Pharmacology and Physiology, 2008, In Press.

45.    Du YH, Guan YY, Alp NJ, Channon KM, Chen AF. Endothelial-specific GTP cyclohydrolase I overexpression attenuates blood pressure progression in salt-sensitive low renin hypertension. Circulation 117, 1045-1154, 2008.

46.    Lin ZY, Chen YF, Zhang WF, Chen AF, Lin SG, Morris M. RNA interference shows interactions between brainstem angiotensin AT1 receptors and ACE2. Experimental Physiology, 2008, In Press.

C.                Ongoing Research Support

1.         NIH/NIGMS R01 GM077352 Chen (PI) /01/2006-05/31/2011  “Redox regulation of endothelial function and wound healing”

2.         NIH/NIHLB   P01 HL70687 Co-I (PI: Fink GD) 07/01/2003 – 06/30/2008 “Neurohumoral control of veins in hypertension”

3.         AHA 0655642Z Chen (PI)  07/01/2006-06/30/2008 American Heart Association      Grant-in-Aid (GIA) “GTP cyclohydrolase I regulation of cerebrovascular function”.

4.         ADA 7-06-IN-01 Chen (PI) 07/01/2006–06/30/2008 American Diabetes Association                Innovative Award  "Endothelial progenitor cell therapy on wound healing in type 1 diabetic mice”.