DMD Highlight: Stable Overexpression of the Constitutive Androstane Receptor Reduces the Requirement for Culture with Dimethyl Sulfoxide
The human liver-derived cell line HepaRG is widely used as an alternative to human hepatocytes for drug metabolism and transporter studies. However, the requirement of DMSO for differentiation of HepRG cells is problematic. Here, the authors demonstrated that stable overexpression of the constitutive androstane receptor in these cells via lentiviral transduction result in increased expression of several P450 enzymes and increased metabolism of low clearance compounds in the absence of DMSO, increasing their value for in vitro metabolism studies.
See the article by van der Mark et al. at Drug Metabolism and Disposition January 2017, 45 (1) 56-67; DOI: https://doi.org/10.1124/dmd.116.072603