DMD Highlight: Comparisons of Stem Cell–Derived Hepatocytes, HepaRG Cells, and 3D Human Hepatocyte Spheroids
To address the need for reliable and versatile hepatic in vitro systems for the prediction of drug pharmacokinetics and toxicity, Bell and co-workers compared transcription profiles and functional readouts of hepatocytes derived from induced pluripotent stem cells, HepaRG cells, and three-dimensional primary human hepatocyte (PHH) spheroids. The authors found that PHHs cultured in 3D spheroids best resemble freshly isolated cells, regarding expression patterns of drug-metabolizing enzymes, drug transporters, and hepatic markers. Consistent with these findings, PHH spheroids could detect cholestatic, carcinogenic, or steatogenic toxicity at clinically relevant drug concentrations.
See the open access article by Bell et al. at Drug Metabolism and Disposition April 2017, 45 (4) 419-429; DOI: https://doi.org/10.1124/dmd.116.074369