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Hosted by the ASPET Division for Molecular Pharmacology (MP)

Wednesday, May 11, 2022, 12:00 pm – 1:00 pm ET

Studying intracellular signaling events has long been a technical challenge. The discovery and application of fluorescent and bioluminescent proteins as research tools has greatly advanced our ability to study, in real time, a range of intracellular signaling events in live cells. One popular method for measuring intracellular events relies on Bioluminescent Resonance Energy Transfer (BRET).  BRET relies on fusing a luciferase onto one protein as a photon donor, and a yellow fluorescent protein on a second protein as a photon acceptor thereby generating an emission as a proxy readout of signaling events in live cells. This method is very versatile and allows for the real time measure of receptor and G protein activation and the production of second messengers in live cells.  This Webinar will discuss the theory behind BRET, what materials and equipment are necessary to perform this method in the average laboratory, and how it can be applied to a broad range of questions in the field of cellular signaling.

Moderators

  • Nina Tsvetanova, PhD, Duke University
  • Angeline Lyon, PhD, Purdue University

Speaker

  • John R. Hepler, PhD, Emory University School of Medicine
    Using Bioluminescence Resonance Energy Transfer (BRET) to Study Cell signaling Pathways in Live Cells

 


Last Updated: May 20, 2022

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